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1 × minimum essential medium– nonessential amino acids (mem neaa)  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1 × minimum essential medium– nonessential amino acids (mem neaa)
    1 × Minimum Essential Medium– Nonessential Amino Acids (Mem Neaa), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1+%C3%97+minimum+essential+medium%E2%80%93+nonessential+amino+acids+(mem+neaa)/pm38229139-253-39-44
    Average 90 stars, based on 1 article reviews
    1 × minimum essential medium– nonessential amino acids (mem neaa) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids.
    Article Snippet: .. On day 0, neuronal induction medium composed of 15% Knockout serum replacement (Gibco), 1% GlutaMax (Gibco), 1% minimum essential media-nonessential amino acids (MEM-NEAA) (Gibco), and 0.1% β- mercaptoethanol (Gibco) in Knockout DMEM/F12 (Gibco) supplemented with 100 nm LDN193189 (Stemgent) and 10 μm SB431542 (Tocris Bioscience) was used. .. On day 2, we changed to the neuronal induction media supplemented with LDN193189, SB431542, 100 ng mL−1 SHH (R&D Systems), and 2 μm purmorphamine (Calbiochem).

    Article Title: Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox.
    Article Snippet: .. Briefly, hiPSCs were cultured in a differentiation medium composed of Dulbecco’s modified Eagle’s medium (DMEM, high glucose, Gibco,Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich Corp., St. Louis, MO, USA), 1 × minimum essential medium– nonessential amino acids (MEM NEAA; Gibco), 1% penicillin-streptomycin (Gibco), and 20% of knockout serum replacement (KSR; Gibco) from D0-D42. .. 10 mM Nicotinamide (Sigma) was added to the differentiation medium at D0-D7, and 100 ng/ml Activin A (Peprotech, Thermo Fisher Scientific) and 3 μM CHIR99021 (Med Chem Express, New Jersey, USA) were added at D7-D14, D14-D42 respectively.

    Cell Culture:

    Article Title: Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox.
    Article Snippet: .. Briefly, hiPSCs were cultured in a differentiation medium composed of Dulbecco’s modified Eagle’s medium (DMEM, high glucose, Gibco,Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich Corp., St. Louis, MO, USA), 1 × minimum essential medium– nonessential amino acids (MEM NEAA; Gibco), 1% penicillin-streptomycin (Gibco), and 20% of knockout serum replacement (KSR; Gibco) from D0-D42. .. 10 mM Nicotinamide (Sigma) was added to the differentiation medium at D0-D7, and 100 ng/ml Activin A (Peprotech, Thermo Fisher Scientific) and 3 μM CHIR99021 (Med Chem Express, New Jersey, USA) were added at D7-D14, D14-D42 respectively.

    Article Title: A novel HDAC8 inhibitor H7E exerts retinoprotective effects against glaucomatous injury via ameliorating aberrant Müller glia activation and oxidative stress.
    Article Snippet: The protein–ligand interactions were generated using BIOVIA Discovery Studio Version 21.1.0. (DS Biovia, San Diego, USA). .. The rat retinal Müller cell line rMC-1 and retinal precursor cell line R28 were both purchased from Kerafast (Boston, USA) and rMC-1 was cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (12100046; Gibco, Carlsbad, USA), while R28 was cultured in low glucose DMEM (11885076; Gibco, Carlsbad, USA) containing a 1% solution of minimum essential medium nonessential amino acids (MEM NEAA) (100×) (11140050; Gibco, Carlsbad, USA). .. For general culture, cells were grown in medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin–glutamine (PSQ) (10378016; Gibco, Carlsbad, USA) in a humidified atmosphere (95% O2 and 5% CO2) at 37 ◦C.

    Article Title: An Engineered IL15 Cytokine Mutein Fused to an Anti-PD1 Improves Intratumoral T-cell Function and Antitumor Immunity
    Article Snippet: .. Ex vivo human T-cell expansion and activation assay Human TILs were cultured in RPMI1640 (Gibco, Catalog No. 21875034) supplemented with 10% human AB serum (Sigma, Catalog No. H4522), 2 mmol/L L-glutamine (Invitrogen, Catalog No. 25030149), 50mM Hepes Buffer (Lonza, Catalog No. 15630080), 1% penicillin–streptomycin (Life Technologies, Catalog No. 15140122), 5 mmol/L Sodium Pyruvate (Gibco, Catalog No. 11360070), and 1% minimum essential medium nonessential amino acids (MEM NEAA; Gibco, Catalog No. 11140050) at 37 C in 96-well flat-bottom plates (5 105CD45þ cells perwell). ..

    Modification:

    Article Title: Tissue engineering RPE sheet derived from hiPSC-RPE cell spheroids supplemented with Y-27632 and RepSox.
    Article Snippet: .. Briefly, hiPSCs were cultured in a differentiation medium composed of Dulbecco’s modified Eagle’s medium (DMEM, high glucose, Gibco,Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 50 μM β-mercaptoethanol (Sigma-Aldrich Corp., St. Louis, MO, USA), 1 × minimum essential medium– nonessential amino acids (MEM NEAA; Gibco), 1% penicillin-streptomycin (Gibco), and 20% of knockout serum replacement (KSR; Gibco) from D0-D42. .. 10 mM Nicotinamide (Sigma) was added to the differentiation medium at D0-D7, and 100 ng/ml Activin A (Peprotech, Thermo Fisher Scientific) and 3 μM CHIR99021 (Med Chem Express, New Jersey, USA) were added at D7-D14, D14-D42 respectively.

    Article Title: A novel HDAC8 inhibitor H7E exerts retinoprotective effects against glaucomatous injury via ameliorating aberrant Müller glia activation and oxidative stress.
    Article Snippet: The protein–ligand interactions were generated using BIOVIA Discovery Studio Version 21.1.0. (DS Biovia, San Diego, USA). .. The rat retinal Müller cell line rMC-1 and retinal precursor cell line R28 were both purchased from Kerafast (Boston, USA) and rMC-1 was cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) (12100046; Gibco, Carlsbad, USA), while R28 was cultured in low glucose DMEM (11885076; Gibco, Carlsbad, USA) containing a 1% solution of minimum essential medium nonessential amino acids (MEM NEAA) (100×) (11140050; Gibco, Carlsbad, USA). .. For general culture, cells were grown in medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin–glutamine (PSQ) (10378016; Gibco, Carlsbad, USA) in a humidified atmosphere (95% O2 and 5% CO2) at 37 ◦C.

    Article Title: Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition
    Article Snippet: .. Spontaneous differentiation was performed by culturing naive ESCs for two passages (4 days) in Dulbecco’s modified Eagle’s medium (DMEM, Corning) supplemented with 15% FBS (Sigma), 1×penicillin-streptomycin (Life Technologies), 1×GlutaMAX (Life Technologies), 1×minimum essential medium nonessential amino acids (NEAA, Life Technologies), and 1×β-mercaptoethanol (Life Technologies). ..

    other:

    Article Title: Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope.
    Article Snippet: MEFs, C3H, and 293T cells were maintained in highglucose DMEM (Gibco) supplemented with 10% FBS (Gibco), 1% penicillin/streptomycin/glutamine cocktail (Gibco), and 1% minimum essential medium nonessential amino acids (NEAA) (Gibco).

    Article Title: Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelope
    Article Snippet: MEFs, C3H, and 293T cells were maintained in high-glucose DMEM (Gibco) supplemented with 10% FBS (Gibco), 1% penicillin/streptomycin/glutamine cocktail (Gibco), and 1% minimum essential medium nonessential amino acids (NEAA) (Gibco).

    Article Title: Preparation of human astrocytes with potent therapeutic functions from human pluripotent stem cells using ventral midbrain patterning.
    Article Snippet: The neuronal induction was performed using a medium with N2:Neurobasal (1:1, Gibco), B27 without vitamin A Invitrogen, Waltham, MA), GlutaMAX (1 %, Invitrogen Fisher Scientific), MEM-NEAA (1 %, minimum essential media-nonessential amino acid, Invitrogen Fisher Scientific), SB431542 (10 lM, Toris Bristol, UK), Noggin (100 ng/mL, Peprotech, Rocky Hill, NJ),insulin (25 mg/L; Gibco) and ascorbic acid (200 lΜ, Sigma-Aldrich).

    Ex Vivo:

    Article Title: An Engineered IL15 Cytokine Mutein Fused to an Anti-PD1 Improves Intratumoral T-cell Function and Antitumor Immunity
    Article Snippet: .. Ex vivo human T-cell expansion and activation assay Human TILs were cultured in RPMI1640 (Gibco, Catalog No. 21875034) supplemented with 10% human AB serum (Sigma, Catalog No. H4522), 2 mmol/L L-glutamine (Invitrogen, Catalog No. 25030149), 50mM Hepes Buffer (Lonza, Catalog No. 15630080), 1% penicillin–streptomycin (Life Technologies, Catalog No. 15140122), 5 mmol/L Sodium Pyruvate (Gibco, Catalog No. 11360070), and 1% minimum essential medium nonessential amino acids (MEM NEAA; Gibco, Catalog No. 11140050) at 37 C in 96-well flat-bottom plates (5 105CD45þ cells perwell). ..

    Activation Assay:

    Article Title: An Engineered IL15 Cytokine Mutein Fused to an Anti-PD1 Improves Intratumoral T-cell Function and Antitumor Immunity
    Article Snippet: .. Ex vivo human T-cell expansion and activation assay Human TILs were cultured in RPMI1640 (Gibco, Catalog No. 21875034) supplemented with 10% human AB serum (Sigma, Catalog No. H4522), 2 mmol/L L-glutamine (Invitrogen, Catalog No. 25030149), 50mM Hepes Buffer (Lonza, Catalog No. 15630080), 1% penicillin–streptomycin (Life Technologies, Catalog No. 15140122), 5 mmol/L Sodium Pyruvate (Gibco, Catalog No. 11360070), and 1% minimum essential medium nonessential amino acids (MEM NEAA; Gibco, Catalog No. 11140050) at 37 C in 96-well flat-bottom plates (5 105CD45þ cells perwell). ..



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